名称:Green Fluorescent Protein (GFP)
来源:Chicken IgY
浓度:10mg/ml
克隆性:多克隆
应用:WB,IHC,ICC/IF,ELISA
推荐浓度:WB:1:3000-5000 IHC:1:1000-2000 ICC/IF:1:1000-2000
Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (RED).
anti-GFP antibody (LEFT panel in RED) and autofluorescence (RIGHT panel in GREEN). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (LEFT), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies as a secondary. The same cell (LEFT) was then identified.
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